Occurrence of hydrocarbon-producing green algal isolates in fresh water bodies of Tamil Nadu
Three samples of green algal isolates were collected and purified from Sivagangai, Tamil Nadu, India. The genomic DNA from the the isolates was isolated using CTAB method and 18S rRNA gene of each isolate was amplified using primers CV1 and CV2. The variation in the DNA sequence was detected through Restriction fragment length polymorphism analysis. The phylogenetic placement of three isolates was investigated using sequences of the nuclear small subunit 18S rRNA gene and the results revealed that the isolated algal strains viz., ALAK 1, ALAK 2 and ALAK 3, were very close to Actinistrum hanzschii, Clamydomonad sp. and Nannochloris sp. respectively.