Helicobacter pylori are the major cause of chronic active gastritis, peptic ulcer disease and gastric carcinoma. Currently, the eradication of H. pylori infection involved a triple therapy, which combines two antibiotic with a proton pump inhibitor. In this study, the aqueous and alcoholic extracts of olive leaf extracts (OLE) and Oleuropeinwere screened for their antibacterial activity against 33 gastric biopsy specimens of H. pylori virulent isolates. Virulent H. pylori isolates were detected by PCR for gene typing of virulent CagA/IceA genes and their distribution among isolates.Resistant to tetracycline and metronidazolewere detected in all isolates (100%), however no resistance was observed to amoxicillin, levofloxacin and clarithromycin. The results of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) indicated that olive leaf extract (OLE) and Oleuropein had a potent antibacterial effect against the virulent H. pylori isolates used in this study. The MICs of OLEs were 50 µg/mL (alcohol extract), 70 µg/mL (cold water extract), and 60 µg/mL (boiled water extract) while the MICs of Oleuropein was ranged between 20 µg/ml to 40 µg/ml. Interestingly, heat treatment of OLE water extract increased its inhibitory activity against H. pylori. MBC of H. pylori genotypes tested was ranged between 60-70 µg/ml for alcohol extract, 70-80 µg/ml for cold water extract, and 80-90 µg/ml for boiled water extract while it was ranged between 50 µg/ml to 60 µg/ml for Oleuropein. These findings suggest that olive leaves extract and its active consecutions Oleuropein is an effective as the commercial antibiotics used against H. pylori infection.