Aim: To determine and compare the antifungal activity of ethanolic extracts of Neem twig and Banyan root against clinical isolates of Candida albicans. Materials and Methods: A total of 20 non repetitive clinical isolates of Candidaspecies were collected from different samples of immunocompromised individuals attending Saveetha Medical college, Thandalam. Characterized by carbohydrate fermentation and assimilation tests and confirmed further using HiChromagar. The ethanolic extracts of Neem and Banyan were prepared. The sensitivity of Candida to the extracts was tested. Results: Of the 20 clinical isolates of Candidaspp, 6/20 (30%) were from oral thrush, 5/20 (25%) from urine, 3/20 (15%) from sputum, 3/20 (15%) from vaginal swab, 2/20 (10%) from ear swab and one (5%) from wound swab. The banyan extract seemed to have greater antifungal effect than neem. Conclusion: Banyan was found to have greater effect than neem. Voriconazole as a standard has a greater sensitivity in comparison with the other two herbal extracts.