Urinary Tract Infections (UTI) are a major cause of morbidity leading to serious long term complications. Profile of uropathogens and the pattern of their antimicrobialsusceptibility vary widely in different geographical regions. The present study was undertaken to determine the bacteriological profile and antibiogram of uropathogens isolated from catheterized and non-catheterized patients at a tertiary care centre. Urine samples were collected from patients with UTI attending a tertiary care hospital. The clean-catch technique of midstream urine was used for patients without catheterization while specimens of catheterized patients (> 48 hours) were collected from proximal part of catheter after aseptic precautions.Profile of bacterial isolates in both the catheterized (50) and non-catheterized (50) groups were almost similar. Commonest organism isolated was Escherichia coli, followed by Klebsiella spp. However, difference in the antibiogram was noted in isolates from both the groups. Our study shows that, E.coli was the most common uropathogen and profile of uropathogens was similar in catheterized and non-catheterized groups. However, difference in the antimicrobial sensitivity pattern in either group emphasizes that different factors play role in determining antibiotic sensitivity of uropathogens isolated from catheterized v/s non catheterized patients. Also, unnecessary catheterization should be avoided and indiscriminate use of antimicrobials in patients with UTI should be discouraged. Urine culture and sensitivity should guide the treatment of UTI in both catheterised and non-catheterised patients.