Evaluation of methodology and comparative study between spin saline tube and matrix gel card techniques for blood compatibility

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Author: 
Dr. Nouratan Singh, Neeraj Singh, Reeba Rachel Joseph, Anil Kumar Gautam and Dr. Neeraj Tandan
Subject Area: 
Life Sciences
Abstract: 

Introduction: A study on Evaluation of methodology and comparative study between Spin saline tube using without AHG, with AHG and Gel card technique for blood cross- matching on the basis of efficacy, sensitivity and specificity was undertaken on approximately 500 samples processed in Blood Bank of U.P. University of Medical Sciences, Hospital, Saifai, Etawah, India. Material and Methods: Most commonly Spin saline tube method are used widely in blood banks. A new technique of cross matching is introduced as AHG gel card. In this study we used Matrix gel card method based on indirect coombs test (ICT) for cross match and tube method including Spin saline tube method with AHG and without AHG. Result: five hundred samples are taken for the study and out of this 490 samples are compatible using Spin saline tube method without coombs reagent, 10 sample shows incompatibility, whereas in Spin saline method by using coombs reagent shows 99.2% compatibility, 06 samples show false positive and 04 samples show true positive of previously result. As per findings specificity and sensitivity is 100% of gel card and tube test using AHG, whereas Spin saline tube test specificity is 98.8 %. Spin saline tube method at room temperature, shows 98% compatibility due to 06- sample false positive and 04 sample true positive, whereas Spin saline tube with coombs reagent at 370C, shows 99.2% compatibility due to 496 sample were found compatible and 04 sample true positive. In matrix gel card also shows 99.2% compatibility. Conclusion: The usage of Matrix Gel card in Blood Bank for cross match is easy to performed with recorded test result and more sensitive and specific then Spin saline tube method whereas indirect coombs tube test is also sensitive and specific but more time consuming as compare to Gel card but cannot recorded result and more time consuming than Spin saline and gel card method.

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