2, 6-dithiol-4-ethylphenol (DTEP) as a photometric reagent for the extractive spectrophotometric determination of Copper (II) is presented in this paper. The reagent DTEP in the presence hydrofobic amins gave instantaneous and stable blue colour with Copper (II) in the pH range 6.2 to 7.7. The Beer’s law was applicable in the range of 0.05 - 4.0 μg/ml at 534-540 nm. The Limit of Detection (LOD) is found to be 8.5-8.7ng/mL. The stoichiometry of the complex is established as 1:1:1 (M: L: Am) by equilibrium shift method. The standard deviation and the coefficient of variance are presented. The results of the prescribed procedure applied for the determination of the micro amounts of Cu (II) in pharmaceutical, food and in plant samples are presented.