Nitrogen (N) is one of the most limiting nutrients in agricultural systems, in this way, the production of high quality soybean requires the nitrogen management in the soil is related to the profitability of the production system. This study aimed to evaluate the yield, seed quality and economic viability of late nitrogen fertilization on soybean.The experiment was conducted in a randomized block experimental design with four replications, with different doses of N: 0, 30, 60, 90 and 120 kg ha-1, in which were conducted two trials (with use of urea and Ammonium sulfate source). The fertilizers were applied at the transition phase between R4-R5. We evaluated vegetative growth components, yield and seed physiological quality and economic viability. The results indicated that the use of urea resulted in higher yield in terms of productivity and with dose of maximum agronomic efficiency of 78.7 kg ha-1N. Urea and ammonium sulfate changed the variables of physiological quality.The economic viability analysis showed difference in gain between sources and between doses, and responses with the use of urea were substantially higher than with ammonium sulfate, and the optimal dose of ureawas 86.7 kg ha-1 N, with net revenue of R$ 8,188. It was concluded that late nitrogen fertilization on soybean provides productive and economic efficiency in seed production system, with lower operational risk with the use of urea as a source of nitrogen fertilizer.