Aerva lanata is consists different kind of secondary metabolites such as steroids, alkaloids, flavanoids, saponind etc., that act as antimicrobial substance to control pathogens. The butanol extracts of Aerva lanata leaves was found all kinds of secondary metabolites. The butanol extract (150 µg/ml) of A.lanata was showed maximum inhibition 5.8 mm against Klebsiella Sp, 5.1 mm against Staphylococus aureus, 4.5 mm against Micrococcus Sp. and 2.6 mm against Pseudomonas Sp. Followed by butanol, hexane, ethanol, chloroform and water showed their antimicrobial activity against test pathogens. A. lanata may be useful to control different antibiotic resistant strains due to presence of active bioactive compounds.