Purpose: Diarrhea remains a major public health problem in the developing world. Diarrhea is an important cause of morbidity and mortality in children. This study investigated the bacterial enteropathogens causing acute diarrhea in Children Under 5 years with special reference to Diarrheagenic E.Coli. Materials and Methods: The study was carried at a tertiary care hospital, children under 5 years of age presenting with acute diarrhea were evaluated. The stool sample of the cases were investigated for parasitic and bacterial pathogens. The E. coli isolated from these samples were tested by molecular method to detect diarrheagenic E.coli. Results: Out of 50 stool samples investigated, 16 (32%) sample yielded E.coli, 5 (10%) were Vibrio cholerae, 3 (6%) were Salmonella species and 2 (4%) were Shigella species. The Giardia species was seen in 5 (10%) cases, 4 (8%) showed eggs of A.lumbricoides and 3 (6%) showed eggs of hookworm and 1 (2%) showed E.histolytica cysts. Mixed infection was seen in 3 cases. Other stool samples showed only normal commensals. All 16 E.coli isolates were negative for diarrheagenic E.coli by molecular method typing. Conclusion: In this study, E.coli was the most common bacteria isolated from stool samples of acute diarrhea cases in children. But caution has to be exercised while reporting it as a pathogen as none of the E.coli isolates in this study were diarrheagenic E.coli. The misinterpretation may result in inadvertent use of antibiotics contributing to drug resistance.