This paper discusses the challenges facing the use of media resources in instruction in the School of Public Health at Moi University. The foregoing study used survey research design to cover the study population of all the lecturers and students. Therefore, 24 lecturers and 213 students were chosen, making a total of 237 respondents. Convenient sampling technique was used to select key informants. Data were collected using questionnaire, interview schedule and observation checklist. The data collected was coded accordingly and analyzed using the Statistical Package for Social Science (SPSS v. 12). The results were presented using descriptive statistics. The findings of the study were that lack of skills and literacy on media resources; high cost of media resources, and media resources not considering students with special needs were the main challenges which influenced use of media resources. Based on these findings, the study recommended that lecturers should be trained on the use of media resources Further research should also be done before adoption of media resources and on e-learning, plus streaming the Media in the School of Public health.