Comparative study of cytotoxic effect by indofil, bavistin and biofungicide on the seeds of lentil (lens culinaris m.)

The effect of three fungicides namely Indofil, Bavistin and biofungicide on mitotic index, chromosomal abnormalities, seed protein content, radicle-plumule length variation on lentil (Lens culinaris Medik) were assessed through a seed treatment experiments. Seeds treated with different doses (0.5%, 1%, 2%) of the three fungicides (above mentioned) solution along with control set. The systemtic fungicides (indofil & bavistin) treated seeds have the ability to induce mitotic abnormalities in large number at 2% concentration, in contrast to biofungicide, which is less toxic. However, the plumule-radicle length is highest in case of seeds treated with 0.5% bavistin & indofil and with 1.0 % in bio fungicide. The endosperm weight decreases gradually in respect to total plumule-radicle weight. In general, the toxicity of fungicide in terms of chromosomal abnormality increases as follows: indofil >bavistin >biofungicide. From the proposed study, it can be concluded that Bio fungicides are more safe than systemtic fungicides as they posed very negligible effect on cell division and chromosome profile.