Evaluation of bioremediation potential of fungal strains for p-nitrophenol

Bioremediation is modern technique in remediation of pollution of environment. Now a days this technique is widely used for remediation of soil and water. According to literature surveysvarious microorganisms have the potential to degrade nitro-aromatic compounds, xenobiotics etc. The white rot fungi are found to excellent organisms for mineralization processes. The purpose of the study is to determine bioremediation potential for PNP by fungal strains. The fungal stains used for degradation assay are (i) Aspergillus flavus NCIM 650, (ii) Aspergillus oryzae NCIM 1212, (iii) Aspergillus niger NCIM 1025, (iv) Fusarium oxysporium NCIM 1008, (v) Rhizopusoryzae NCIM 997, (vi) Scleratiumrolfssi NCIM 1048 and (vii) some agriculturally important microbes including Aspergillus awamori, Trichoderma sp. About 100 ml of sterile MSG medium was supplemented with filter sterilized PNP at 50 ppm concentration was taken in Erlenmeyer flask. This medium was inoculated separately with above fungal strain and incubated on a rotary shaker (120 rpm) at room temperature. The flasks were monitored for 8 days for the disappearance of yellow colour of PNP. After 8 days, the each fungal culture was renioculated in fresh sterile MSG medium with 50 ppm PNP. After five repeated enrichment for 5 weeks, each flask was observed for disappearance of yellow colour.