Gloriosa superba L. is associated with a plethora of medicinal benefits and ethnomedical applications since time immemorial. The current rare state of this plant is the result of over-exploitation for its medical uses. To address Gloriosa superba's low seed set issue, tissue culture and somatic embryogenesis techniques needed to be standardized. In the present study, we tried to develop a standard protocol for in-vitro propagation of G. superba in the Terai region of Uttar Pradesh. G. superba was cultured under circumstances designed for in vitro regeneration utilizing nodal segment explants. The combination of MS+1.5 mg/l BAP+0.5 mg/l NAA produced the most number of shoots (7±0.18), demonstrating the best shoot induction. The number of shoots per culture increased by adding 10% coconut water (CW) to the previously mentioned medium, and the length of the shoots increased by adding 100 mg/l of urea to the media. Shoots cultivated on half-strength MS supplemented with 1.0 mg/l IBA + 0.5 mg/l NAA showed the best rooting. The medicinal and conservation components of G. superba will benefit from this established approach, enabling it to satisfy future economic demands.
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