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Analysis of genetic diversity in five genotypes of cicer based on protein profiling and rapd

Author: 
Jahangir, A.D., Vaseem, R. and Kudesia, R.
Subject Area: 
Life Sciences
Abstract: 

Electrophoretic SDS-PAGE and RAPD analyses were performed to estimate genetic diversity in five Cicer cultivars. In SDS-PAGE, a total 36 polypeptide bands were detected, only 4 were monomorphic and the rest 32 were polymorphic with an average polymorphism of 88.88%. Jaccard’s similarity ranged from 0.25000 to 0.56000. A dendrogram constructed based on UPGMA clustering method revealed two major clusters, cluster 1 and cluster 2, comprising of two accessions each. The accession Shubhra occupies a distinct place as revealed in the dendrogram. In case of RAPD, primer OPM-05 was used and led a total of 21 bands, all of them were polymorphic. Dice’s coefficient ranged from 0.00000 to 0.25000. A dendrogram constructed based on UPGMA revealed two major clusters. Accession DCP-92-3 and IPC-04-20 are genetically dissimilar, hence it is recommended that these should be used for future breeding programs to create higher amount of genetic variability in chickpea.

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