The present study was under taken to investigate the antibacterial and antifungal activity of seeds of Jatropha curcas Linn. The powdered seed materials were extracted using chloroform and hexane solvent. The antimicrobial activity against Staphylococcus aureus, (gram positive bacteria) Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhimurium (gram negative bacteria) and fungi Candida albicans, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Rhizopus sp., and Mucor sp. The antibacterial and antifungal activity in vitro using the disc diffusion method, Minimum Inhibitory Concentration (MIC), Minimum Bacterial Concentration (MBC) and Minimum Fungicidal Concentration (MFC) is discussed.