This plant evaluates the antifungal activity of methanolic extract of Putranjiva roxburghii Wall. of Euphorbiaceae. For centuries plants are used as food and medicine. The present study focuses on the antifungal activity of leaves of P. roxburghii. Strains of Aspergillus candidus, Chrysosporium tropicum, Rhizopus stolonifer, Microsporum canis and Trychorphyton rubrum are the fungal strains used to check the mode of action. Methanol leaf extract (sample1) showed highest activity against M. canis strain than methanol bark and methanol root extracts. Methanol bark extract (sample 2) showed more activity against T. rubrum strain, here R. stolonifer and A. candidus showed very similar range of activity against fungi. Methanol root extract (sample 3) showed more activity against T. rubrum strain. Along with other properties these plant extracts also have antifungal property.