Lawsonia inermis plant, generally identified as Henna, is a medicinal plant and leaves of this plant are used as dye from ancient time. The present study was conducted to study antityphoid activity and phytochemical screening of different extracts of Lawsonia inermis leaves. Methanol extract showed highest inhibition zone (13.74±1.52) at 20mg/disc and lowest inhibition zone (8±1) was demonstrated at 5mg/disc of hexane extract. Phytochemical screening of different extract revealed the presence of various phytoconstituents such as flavanoids, alkaloids, carbohydrates etc. Quinone is the main phytoconstituents which is responsible for antityphoid activity and presence of this compound was confirmed in all extracts while protein was absent in all extract of Lawsonia inermis plant leaves.