Application of salmonella typhi’s outer membrane (omp) in diagnosis of typhoid

In this study, a modern technique for the diagnosis of Salmonella typhi using the outer membrane protein was established. To reduce the identification time of the infection by typhoid fever; Salmonella typhi outer membrane protein was extracted from Salmonella typhi bacteria ATCC 19430 growing on optimized growth conditions. Partially purified outer membrane protein was immobilized on a nitrocellulose membrane and tested against serum of typhoid patients to detect the antigenicity of the protein isolated using immunoblotting technique. The molecular weight of the highly antigenic protein was measured and it was about 50KDa. These antigens showed high antigenisity against the sera of Typhoid fever patients using Western blotting technique. So, this can be an initial step towards the rapid immunochromatographic strips and ELISA diagnostic kits for the efficient detection of antibodies against Salmonella typhi in serum specimens.