The basic objective of this research was identifying and discussing the obstacles that affect knowledge sharing among Assosa university academic staffs. Descriptive survey research design method was used to achieve the objective of the study. The target population of the research was all Assosa university academic staffs including faculty deans. Questionnaires were used to collect data from the academic staffs and interviews were also used to collect data from the faculty deans. Data was analyzed using qualitative and quantitative methods. Descriptive statistics (frequency and percentage) were used to analyze quantitative data. The study attempted to assess the knowledge sharing mechanisms and practices of ASUAS, knowledge sharing obstacles ASUAS and the models of KS. The results or findings of the study showed that there were obstacles that prevent knowledge sharing among academic staffs; such as lack of ICT infrastructures, lack of knowledge storage mechanisms, The lack of trust among academic staffs, lack of incentives or rewards for those who share knowledge for others, Lack of interaction between those who need knowledge and those who can provide knowledge among ASUAS, physical layout of work areas (office arrangement of staffs) and ASUAS culture.