Rate of oyster spat fall was recorded in Mulky estuary(a tropical estuary), south west coast of India from October 2009 to May 2010. The average rate of spat fall ranged from 24.96 to 1058.43 No/m². The spat fall rate was found to be higher on concave surface than convex surface of the oyster shells set for studying spat fall rate. The most suitable period for oyster spat collection in Mulky estuary is from December to January and April to May of the year. January and April appeared to be the months of peak settling. Therefore, oyster farmers may set their clutch materials during these periods to collect maximum spats for culture practices. Several environmental factors influence on the settlement of oyster spat. There was a significant positive correlation between oyster spat fall rate and water temperature, salinity, DO and pH.