
The present study was carried out to investigate the seasonal variations of moisture, ash protein, lipid and fatty acid (per cent) of muscle and liver of adult male and female Labeo rohita. For this purpose seasonally ten live specimen of both sex ranged in total length 34.5 to 42.5 cm. and weight 950gm to 1200gm were considered during study period (2008 to 2010). The amount of protein, lipid and fatty acid level increase during spring whereas moisture content of muscle and hepatic organ increases during summer and rainy season. Seasonal variations of lipid content followed the opposite pattern of the water content. The mean values of these biochemical constituents varied significantly (p<0.05) in different season. There was significant negative correlation between moisture and lipid (r=-.582*), positive correlation between ash and protein(r=.868**), protein and lipid (r=.966**). Reduced biochemical parameters coincide with that of gonad development and breeding period of the species in the rainy season (p<0.01). The female specimen contained more moisture and lipid whereas more protein was found in male. ANOVA was applied to assess the difference among various biochemical parameters. Significant differences were observed in these fish tissues which indicate the quantitative and qualitative nature of fish sample for nutritive purposes.