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Cloning of a Gene Encoding Testis Specific Serine/Threonine Kinase 3 from Mus musculus

Author:

Gnanaraj, M., Kowsalya, R., Padmavathi, K. and Elangovan, N.

Subject Area:

Life Sciences

Abstract:

Testis specific protein kinases are important because of their potential role in spermiogenesis, sperm maturation and sperm function. To continue our studies on the testis specific serine/threonine kinase (TSSK) family, we isolated a PCR fragment encoding a member of testis specific serine/threonine kinase3 (TSSK3) from Mus musculus testis RNA using reverse transcription polymerase chain reaction (RT-PCR) with oligonucleotides. The sequence length of the target gene was identified as 804 bp encoding a protein of 268 amino acids.

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