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Colorimetric estimation of prasugrel in bulk and pharmaceutical formulations

Author: 
Paramdeep Bagga, Mohammed Salman Khan, Mohammed Haris Siddiqui, Tariq Mahmood and Hefazat Hussain Siddiqui
Subject Area: 
Health Sciences
Abstract: 

A simple and cost effective colorimetric method is described for the determination of prasugrel in pure form and in pharmaceutical formulations. The determination of the drug in pharmaceutical formulations is based on the drugs redox reaction with Folin-Ciocalteu reagent (Phenol reagent). The linearity range for prasugrel of blue chromogen produced at wavelength of detection 725 nm was obtained as 10–50 µg/ml. The linear regression equation obtained by least square regression method, were Y =0.0173.X – 0.1671, where Y is the absorbance and X is the concentration (in µg/ml) of pure drug solution. The absorbance was found to increase linearly with increasing concentration of prasugrel, which is corroborated by the calculated correlation coefficient value of 0.999. The limit of detection and limit of quantification was found to be 1.6145µg/ml & 4.8925 µg/ml respectively. The validity of the described procedure was assessed. Statistical analysis of the result has been carried out revealing high accuracy and good precision. The proposed method was successfully applied to the determination of prasugrel in pharmaceutical formulations without any interference from common recipients.

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