Medicinal plants are important sources for isolation of pharmaceutical drugs. The current available drugs are in many ways either inefficient or unaffordable to ever increasing forms of microbial infections. Screenings of medicinal plants used by the traditional medicinal healers are the main sources for formulation of herbal drugs. Phytochemicals present in plants are economically important sources of drugs, fragrances, pigments, food additives and pesticides. The present paper reports the analysis of qualitative, quantitative and antimicrobial properties of Centella asiatica L., Lawsonia inermis L. and Eclipta alba L. Centella asiatica was found to have maximum contents of Flavonoids and phenolics whereas Lawsonia inermis has maximum contents of tannin. Antimicrobial analysis study showed that Centella asiatica has maximum antibacterial property against Escherchia coli MTCC68 and the plant extract of Eclipta alba showed maximum antimicrobial property against Staphylococcus aureus MTCC3160. The present work will help in understanding the comparative phyotochemical and antimicrobial properties of Centella asiatica, Lawsonia enermis and Eclipta alba. Centella asiatica, and set forward towards isolation and characterization of the bioactive compounds present in these medicinally important plants.