Comparative morphometric assesment and protein profiling of fasciola hepatica and fasciola gigantica coexisting in bovines

Fascioliasis is one of the most prevalent helminth infections of ruminants in different parts of the world. The current study highlights the phenotypic differences of aetiological agents of Fascioliasis in bovines i.e., Fasciola hepatica and Fasciola gigantica. The phenotypic parameters taken into consideration were BL, BW, OL, OW and BL/BW ratios. The data was subjected to one way ANOVA followed by Tuky test by using PRIMER version 4. Morphometrical values of Fasciola spp. revealed longer F. gigantica (33.66 4.42) as compared to Fasciola hepatica (25.194.22). Moreover, F. gigantica had narrower bodies (5.48 ) compared to F. hepatica (5.70 ). The differences in the mean body length; mean body width; and mean of BL/BW ratios of body were significant (p<0.05). The current abattoir study also revealed the predominance of F. gigantica (78.16%) to F. hepatica (21.39%) in cattle. The electrophoretic pattern under reducing conditions of 12% SDS-PAGE showed some similarities and differences between crude somatic protein extraxt of Fasciola gigantica which revealed presence of 11 bands and 14 bands in case of Fasciola hepatica coexisting in bovines.