The objective of this investigation is the comparative study of antibacterial and antifungal potentials of citric acid and tartartic acid based nano composites on the growth inhibition of some important pathogenic fungi and bacteria in vitro. The study is to assess the antimicrobial activity and to determine the zone of inhibition of nanocomposites on some bacterial and fungal strains. In the present study, the microbial activity of (Tartartic acid + Glycerol + n-HAp) TAGH and (Citric acid + Glycerol + n-HAp) CAGH nanocomposites was evaluated for potential antimicrobial activity against medically important bacterial and fungal strains such as Staphylococcus aureus, Bacillus subtilis, Salmonella typhi and fungi namely Candida albicans, Trichoderma viride, Rhizopus microspores. Antibacterial activity of the extracts was determined by disc diffusion method on Muller Hinton agar (MHA) medium. Antifungal activity of the extracts was determined by disc diffusion method on Sabouraud Dextrose agar (SDA) medium. The assay was performed by agar disc diffusion method. The results demonstrated that citric acid has more bactericidal and fungicidal activities than those of tartaric acid against all pathogenic bacteria and fungi tested. The small size of the Nanomedicine is very suitable for carrying out antimicrobial biological operations. Further research is needed to assess the efficacy of citric and tartaric acids as inhibitors of bacterial and fungal growth in clinical trials, especially in treatment of patients with microbial infections.