Comparative study on the spectrophotometric determination of some selected 5-nitroimidazoles

The present study is aimed at making a comparative study of the direct spectrophotometric determination of 5-nitroimidazoles such as metronidazole, ornidazole, tinidazole, secnidazole and satranidazole with 0.5% sulphanilamide and 0.3% NEDA. Each of the drug samples was boiled for 90 minutes at a temperature of 90oC followed by the treatment with the reagents 0.5% sulphanilamide and 0.3% NEDA. It exhibited a stable instantaneous reddish purple, colour, which showed maximum absorbance at 540nm. With each of the above mentioned drug samples, the same colour was observed and the maximum absorbance was at 540nm for all. Beer’s law obedience, correlation factor, stability, LOD and LOQ were given below for a comparative study.