Detection of (meca)gene in methicillin resistant staphylococcus aureus (mrsa) from outpatients clinic in baghdad hospital, iraq

Total (230) isolates of Staphylococci species were isolated from different clinical samples. They were distributed isolates from urine, wound infections and ear swabs. These isolates were diagnosed using different morphological and biochemical test. Out of 100 isolates were Staphylococcus aureus. Disk diffusion method antibiotic susceptibility were used, 13 methicillin resistant S. aureus isolates was identified, It was found that 13(100%) isolates have positive result for mecA gene expressed S.aureus (mecA)in their PCR products. It was concluded that the polymerase chain reaction appears to offer a time saving and accurate method of detection of methicillin-resistant S.aureus. and it can be used to detect a lowlevel of it from different samples without using a selective medium or additional biochemical tests.