Aiming to increase the yield and quality of tomato and pepper fruits, alternatives are sought, such as the use of biostimulants applied to the seed. The objective was to evaluate the effect of the application of different doses of a biostimulant on the physiological quality of tomato and pepper seeds. The research was carried out in the period between March and July 2016 in the Laboratory of Seed Quality Analysis of the Faculty of Agrarian Sciences of the National University of Asunción. The experimental design was completely randomized in a factorial scheme 2x4, with four replications for each treatment, where factor A corresponded to the species and factor B corresponded to the doses used, totaling 32 experimental units. The variables studied were germination (%), germination rate, germination rate index, germination rate coefficient, root length, stem length and emergence. The collected data were subjected to analysis of variance and in the case of significant statistical differences, Tukey’ stest for comparison of meanswas performed at 1% error probability. The results showed that for tomato, no significant differenceswere observed in the variables germination and emergence, nevertheless, there were differences between the other variables. Likewise, for pepper, no statistical differences were observed regarding the emergence, in contrast with whatwas verified for the remaining variables. It is concluded with this work that the biostimulant application does not influence the physiological quality of the tomato and pepper crops.