The present attempt has made to study the diversity of zooplankton with special reference to quality and quantity from vadgaon freshwater reservoir from Kolhapur district of Maharashtra, India. The study revealed that 16 species of zooplankton were noted belonging to 4 groups namely, rotifers, cladocers, copepodes and ostracods. Qualitatively, roifers and cladocers were observed dominant while quantitatively rotifers were dominant. All four groups have shown monthly variation during both the years and noted lower during monsoon season while higher during winter as well as summer season. Cladoceran group was an only exhibited its presence throughout the study period. Based on the present investigation it can be concluded that the water from the reservoir is clear and pollution free.