This study was designed in such a way to assess the toxicity effect of 2,4- D (Dichlorophenoxyacetic acid) pesticide in fish physiology and its antioxidant stress. Channa striatus fish were administered with two different doses compensation of 2,4-D pesticide of 100 and 200 mg/kg. After a matter of time interval the effect of 2,4-D on fish behavior and its toxic level was determined by assessing its blood parameter and antioxidant enzyme level. Hemocyte count has considerably reduced in 2,4-D treatment and its responsive antioxidant enzyme level of Superoxide dismutase (SOD) and Catalase level was also drastically altered. The present study concludes the toxic effect of 2, 4-D usage.