The present study was carried out to investigate the effect of fruit pulp extract of Carica papaya (seeded and seedless fruits) on male albino Wistar rats. The experimental animals were divided into three groups, One group served as control, Group- II , received papaya pulp extract from seeded fruits and Group - III received papaya pulp extract from seedless fruits. Both groups were orally administered with pulp extract (10ml/kg/body weight) for 60 days. Pituitary gonadotropins (LH, FSH) and steroid hormone (testosterone) analysis was carried out on 21st, 41st and 61st day. There was a significant decrease (p<0.05) in the concentration of LH, FSH and testosterone on the 20th, 40th and 60th day. These results may indicate that Carica papaya fruit pulp extract of seeded and seedless possess antifertility adverse effects.