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Effect Of Cultural And Environmental Parameters On Growth And Cellulase Production Of Streptomyces Species Isolated From Municipal Wastes

Author: 
Prasad, P., Bedi, S. and Tanuja
Subject Area: 
Life Sciences
Abstract: 

This investigation intended to comparatively study and evaluate independent capacities of cellulose degrading native strains of Streptomyces albospinus (MTCC 8768) and Streptomyces somaliensis (MTCC 8769) isolated from municipal wastes of Patna for growth adaptability and endoglucanase yield, divulging a new insight for a strong suitability of these isolates for large scale production. Both the isolates were able to grow well in all the media tested. However, S. albospinus (MTCC 8768) showed luxuriant growth as compared to S. somaliensis (MTCC 8769) indicating its wider substrate utilization capacity. The isolates were also able to utilize different carbon sources with exception of lactose. Maximum cellulolytic activity was observed at 37 °C using Congo red test that exhibited a clear zone of 4 mm by S. albospinus (MTCC 8768) and 3 mm by S. somaliensis (MTCC 8769) around the colonies. Optimum pH was 6.8 at which the amount of reducing sugar released was 9.2 mg/ml by S. albospinus (MTCC 8768) and 8.3 mg/ml by S. somaliensis (MTCC 8769). S. albospinus (MTCC 8768) showed a better adaptability for different media and carbon sources that makes it more suitable to be used for commercial production of the enzyme.

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