A large number of pesticides are commonly used to control various agricultural pests; however, their toxicological impact also extends to non-target species like fish. Fish is good indicator of aquatic contamination because its biochemical stress responses are quite similar to those found in mammals.The aim of the present study is to assess the glycogen content in brain and muscle of the fish Channa striatus exposed to sublethal concentrations of endosulfan 1/10th (high), 1/15th (medium) and 1/20th (low) of the 96 hour LC50 values for the period of 7, 14 and 21 days. The fish exposed to endosulfan showed a decrease the glycogen level for 7, 14 and 21 days in brain and muscle. However, no information is on record concerning the three different sublethal concentration of endosulfan on the glycogen contents of fish. The objective of the present work was to observe the effect of endosulfan on glycogen levels in brain and muscle of freshwater fish, Channa striatus.