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A field study was carried out at college farm, College of Agriculture, Rajendranagar, Hyderabad, Andhra Pradesh during Kharif 2013 to find out the influence of humic acid and biochar on soil enzymes of maize rhizosphere at different stages. The experiment was laid out in a Randomized Block Design and replicated thrice with three factors comprised of factor-I (fertilizers- 100 % RDF and 75 % RDF), Factor-II (biochar levels- 0, 5 and 7.5 t ha-1) and Factor-III (humic acid levels of 0 and 30 kg ha-1). Dehydrogenase activity at 30 DAS was significantly higher the activity being 16.19 μg TPF g-1 soil day-1 with 75% NPK in combination with humic acid. While, at 60 DAS and harvest, significantly higher activity was noticed with biochar @ 5 t ha-1 along with 100 % NPK and 75% NPK respectively with the corresponding activities of 20.72 and 5.594 μg TPF g-1 soil day-1. Combined application of 75% NPK, 7.5 t ha-1 of biochar and 30 kg ha-1 of humic acid could result in a significantly higher acid phosphatase which was on par with recommended NPK along with biochar @ 7.5 t ha-1 at all the stages of assay. Alkaline phosphatase activity highest at 30 and 60 DAS (48.93 and 158.6 μg p-nitro phenol g-1 soil h-1) with 100 % NPK along with biochar @ 7.5 t ha-1, while at harvest stage higher activity (37.31 μg p-nitro phenol g-1 soil h-1) was shown by 75% NPK alone.
Rosane Cavalcante Fragoso, Brasil
Chief Scientific Officer and Head of a Research Group
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