Effect of growth regulator on in vitro organo genesis of Prosopis cineraria (L.) Druce

Prosopis cineraria L. is a species of flowering tree in the pea family, Fabaceae. It is the state tree of Rajasthan (India). Due to its economic importance in state Rajasthan has drawn attention towards it. In the present study, the propagation of a multipurpose tree species Prosopis cineraria under in vitro condition. Binodal explant from the mature plant of Prosopis cineraria was taken and cultured after surface sterilization. The explants were cultured on MS medium supplemented with various concentration of cytokinins and auxins individually and in various combination (1.0–3.0 mg/l) BAP, Kn, 2,4-D. Maximum percentage of callus induction were recorded at 2.5mg/l 2,4-D with 0.5mg/l Kn. Callus is directly sub cultured on MS medium having different concentration of auxins and cytokinin:(0.5 – 5.0 mg/l) BAP, Kn, α-NAA and 2,4-D. Maximum shoot induction was observed on 2.0mg/l BAP with 1.0mg/l α-NAA.