Chromium is present in trace in all organic matter and seems to be an essential mineral. Chromium plays an important role in the metabolism of carbohydrate and fats. It works with insulin in the metabolism of sugar. It seems to increase the effectiveness of insulin, thereby facilitating the transport of glucose in to the cells and not allowing the blood glucose levels to rise. It helps to take protein where it is needed and also an aid in growth. Chromium has been found beneficial in the prevention and also an aid in growth .Chromium has been found beneficial in the prevention and treatment of high blood pressure. In spite of its uses it also causes severe impairments in animals including man. It is available in excess ie., above the normal level. Hence, in the present study the impact of sub lethal concentration of hexavalent chromium compound (Potassium dichromate) on total carbohydrate ,glycogen and total free sugar was estimated in the tissues such as brain, liver, kidney and muscle of Newzealand white rabbit exposed to 1,5 and 9 months duration was carried out. The results shows the total carbohydrates, total free sugars and glucose increased when compared to the normal rabbit. The glycogen gradually decreased. The results are discussed, the values are statistically significant.