Introduction: Sedentary individual are more prone to have the muscle tightens, sedentary individual are the individual who does lot of activity in sitting postures. Hamstring muscle is a two joint muscle. Hamstring flexibility should be maintained well to gain full range of motion in both the joints. During ambulation activity of hamstring are more important. Stiffness in the hamstring could cause postural imbalance, pain, disability and reduced stability in the joint. Aim of the study is to identify the efficacy of Dynamic Stretching with Muscle energy technique on Hamstring flexibility in Sedentary Individuals. Quasi experimental study with 30 sedentary individuals was selected and their hamstring length was measured sit and reach testing. 30 volunteers were randomly allocated into two groups, experimental group with muscle energy technique and the control group with dynamic stretching. The programme was conducted for 8 weeks and the values of the hamstring flexibility were measured using sit and reach test. The pre value, 4th week and 8th week values were taken for the analysis. ANOVA was used to analyze the difference between the durations and the Tukey HSD test used to identify the amount of differences in each groups. Results: The result of the study shows that there was a significant improvement in the experimental group when compared with the control group with the significance of p value at 0.05. The Anova value is 56.7 in the Muscle energy technique group and the value is 168.54 in the dynamic stretching group. The critical value of U at p < .05 is 64. Therefore, the result is significant at p < .05. This study concluded that MET stretching would produce more hamstring flexibility when compared to the dynamic stretching in hamstring tightness.