Spermadictyon suaveolens Roxb.is an ancient Indian herb used as a various medicinal products. In present investigation the callus induction of Spermadictyon suaveolens Roxb. were carried out by using Murashige and Skoog (MS) medium with different concentration of hormones. The explants, collected from the Matwan gaon, Taluka Dapoli, District Ratanagiri (Maharashtra) and grown on the garden of Department of Botany, University of Pune. The leaf explant respond well for callus and cell culture biomass by using MS with 1.2 mg/l TDZ.