An investigation was undertaken to isolate poly β hydroxylbutyrate (PHB) producing bacteria from Tannery waste sludge reservoir In Mario Jose Kano. A total of six isolates were purified, only three sp were selected for the production of PHB viz: Alkaligen sp Bacillus megategarium and Clostridium sp. PHB extraction was carried out by sodium hypochlorides digestion method. The effect of mutant on different bacteria using different UV – light radiation exposure were carried out on three bacteria sp base on time duration (5,10, 15, 20 and 30 min) which shows a significant reduction of growth with increase in time of UV light exposure. The culture conditions have been investigated at optimum conditions. Alkaligen sp and Bacillus megaterium were found to be most efficient PHB producers. Maximum production of PHB yellow purple "Dorowa” (Pakia biglobosa) was shown by Alkaligen sp (2.02 g/g using dried cell weight at 2.80 g/l) and Bacillus megaterium (1.52 g/g using dried cell weight at 1.93 g/l) yield and dried cell weight respectively. Thus, the present data indicate that yellow purple (Pakia biglobosa) could be alternatively used for PHB production. Use of bioplestic produced by these organisms, will help to reduce environmental pollution.
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