In the present article an attempt has been made to analyze large scale production of tobacco mixed gutkha and panmasala by several spectroscopic techniques. In the Asian countries like India the materials used by the peoples at the extreme level as their regular diet. Due to their regular consumption and addict numerous disadvantage has been come out in the light. Number of research article flaunting that in the materials like tobacco and panmasala (Raj Shrestha Ashwini Kumar et al., 2012) such as rajngandha etc. trace amount of heavy metals and transition metals are present, presence of these metals has adverse effect on human health. This article summarizes recent efforts to estimate and amount t hazardous substances present in these materials (1, 2). In primary investigation the metals present in tobacco and panmasala (1, 2) were quantitatively estimated, then the metals were characterized by AAS. Some organic substances are estimated by using HPLC. Tobacco is a plant within the genus Nicotiana of the Solanaceae (nightshade) family. Harmful effects of tobacco consumption can derive from the thousands of different chemicals in the smoke, including cadmium nickel, arsenic, iron, zinc many other transition and heavy metals. The heavy metals were estimated by using AAS from both samples (1,2). HPLC data used for the characterization of samples and indicates presence of organic hazardous chemicals including polycyclic aromatic hydrocarbons (such as benzopyrene), formaldehyde, tobacco-specific nitrosamines (TSNAs), phenols, and many others (Supadminidevi et al., 2012). Tobacco also contains beta-Caroline alkaloids.