During the last few years it is noticed that everybody is getting concern about natural dyes. Mainly the sources of natural dyes have three categories viz. plants, minerals and animals, but plants take great role for obtaining the natural dyes. Due to its less pollution and less side effect, natural dyes are used more often in food products as well as other important regular uses. Methanolic extract of different seven dye yielding plant parts like Acacia catechu (L.f) Willd., Basella alba Linn. var. rubra Linn., Beta vulgaris Linn, Bixa orellana Linn., Carthamus tinctorius L., Terminalia arjuna (Roxb.) Wight. & Arn., Terminalia chebula Retz. Were used to investigate the antimicrobial properties and antimutagenic activity. The methanolic extract of the plant parts are tested by agar well diffusion method (AWDM). The investigate showing the positive response against one gram +ve bacteria and three gram –ve bacteria. The result showing the positive response against the all microorganism. The tested plant parts showing the varying level of antimutagenic activity. The concentration dose 25, 50 and 100 µg/plate of dyeing parts of methanolic extract are widely used for this investigation. It was found that the dyeing parts of methanolic extract of the plant a dose 100 µg/plate exhibit inhibition of His+ revertants against acting mutagens, sodium azide (NaN3) induced mutagenicity in Salmonella typhemuium tester strains TA98 and TA100. The over all result showed that the tested methanolic plant extract both have antimicrobial properties and antimutagenic activity. Enumeration includes details about the plant with prospecting proper medicinal value.