Free radical mediated oxidative stress is believed to be the primary cause of many disorders, such as cardiovascular diseases, cataract, arthritis, brain dysfunction, diabetes mellitus, cancer, ageing etc. In treatment of these diseases, antioxidant therapy has gained an utmost importance in the resent years. Current research is now directed towards finding naturally occurring antioxidants of plant origin. Andrographis echioides and Boerhavia diffusa are important medicinal plants, which have a wide range of application. In the present study, the antioxidant potential of ethanolic extract of Andrographis echioides and Boerhavia diffusa was evaluated by determining the levels of enzymatic and non-enzymatic antioxidants. Our results showed that both the plant extracts possessed significant levels of enzymatic and non-enzymatic antioxidants. However, Andrographis echioides showed higher levels of enzymatic and non-enzymatic antioxidants than Boerhavia diffusa.