Evaluation of genetic diversity of the important medicinal plant crab’s eye (abrus precatorius l.) using rapd and issr markers

Genetic relationships between five genotypes (Abrus Precatorius. L) with different seed coatcolours (Red with black eyespot, black, white brown and maroon) were collected from different places of Andhra Pradesh were analyzed using RAPD and ISSR markers. Ten RAPD (decamers) and five (ISSR) primers were prescreened for their amplication potential. Of which 3 RAPD and 3 ISSR primers produced clear and reproducible amplified products. For RAPD analysis, 3 random primers (OPA – 02, OPA – 16 and OPC – 09) were used which amplified 25bandsof which 15 were polymorphic with and ever age polymorphism of 60.00%. the amplified products varied size from 500 – 3000 bp. For ISSR analysis, 3 primers were used which produced 32 bands, 23 of which were polymorphic (71.87%). The size of amplified bands ranged from 500 – 3000 bp. The efficiency of primers in generating sufficient information for genetic diversity analysis was computed using discriminatory power (Di), which ranged from 0.00 – 0.24 for RAPDs and 0.00 – 0.28 for ISSRs. The date analysis based on Jacquard’s similarity coefficient and UPGMA Clusters analysis revealed that genotypes with Red and brown on seed coats were more diverse as compared to other three genotypes (RAPD Clusters) while as in ISSR cluster analysis. Maroon and red seed coats were more diverse as compared to black. Because maroon and coated genotype was collected from Seshachalam Forest, which is located inChittor District, while the rest with four genotypes were collected from east Godavari district, Andhra Pradesh.