The quality of human use water at the point of delivery to the peoples houses is related directly to the consumer's health. The current study assessed the changes in residual chlorine content with distance in water distribution system in Abu-Alkhaseeb district, Basrah city, South of Iraq. Collection of water samples have been done from the start point of the water treatment plants, mid distance and the taps at consumer households at an intervals of 1-2 km. Standard methods were used for determination of residual chlorine contents and present of total and fecal coliforms of 36 water samples collected from water pipeline distribution systems related to 12 water treatment plants. The mean concentration of the residual chlorine from all sampling stations was between (0-3.5). Total coliforms detected in the most water samples in the range of (0-34) cfu/100ml while fecal coliforms were in the range of (0-20) cfu/100ml. The chlorine concentration levels in the transported water were decreased with the time and distance. The results indicate strongly that the residual chlorine in most sampling locations is not sufficient to support the elimination of bacterial growth in the water distribution system unless the specialists repair the water pipe leakages and other damages which are the main source of the bacterial contamination and focus on careless of the responsible staff in order to treat the water with accurate chlorine concentrations.