
In this study, Phenylalanine ammonia lyase (PAL) was evaluated as one of the important enzymes in the pathway of phenylpropanoids synthesis in order to understand the structural fold and sequence homology; so that it was extracted from Protein Data Bank (PBD): X-ray crystallographic structures of Rhodosporidiumtoruloides (1T6J) as a genus of fungi category and (1W27) Petroselinumcrispum as a genus of plants category. In order to structural analysis of PAL has been used some software include Protein Structure Comparison Tool V 1.4, on-web PDBe version based on secondary structure matching (SSM) and Combinatorial Extension(CE) for Structure Alignment, calculation of statistical indicators such as Z-score, p-value, Q-score and etc. Finally PAL showed a significant relationship between structural sequences of 1T6J & 1W27 in terms of structural folds similarity and partialhomology with calculated indices between the two enzymes.