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Evaluation Of Phytochemical, Phytonutrient And Thin Layer Chromatography Profiling Of Sequential Extracts Of Vernonia Cinerea

Author: 
Uma Ramaswamy and Vicky Mani
Subject Area: 
Physical Sciences and Engineering
Abstract: 

Vernonia cinerea is a annual herb which is commonly found in India especially in South India. The aim of the study was to evaluate the phytochemical, phytonutrient (carbohydrates, protein, lipid, vitamin,minerals, ash, moisture) and thin layer chromatography profiling of the sequential extracts of whole plant V.cinerea. The sequential extract of acetone, ethanol, ethylacetate and aqueous extract of V.cinerea for analyzing phytochemical and confirmed by thin layer chromatography (TLC). The phytochemical result depicts the presence of various bioactive compounds which includes phenolics, saponins, steroids, alkaloids, flavonoids, terpenoids, tannins and cardiac glycosides. The phytonutrient report shows total protein content was found to be higher (42.6 mg/g) than lipid(11.6 mg/g) and carbohydrate(1.62 mg/g) respectively. Among the minerals quantified calcium content was higher than phosphorus and iron. Riboflavin was found to be present in lesser amount when compared to vitamin thiamine,tocopherol and ascorbic acid.TLC profiling of all the extracts of V.cinerea confirms the presence of phytochemicals and different Rf values of compound reflects an idea about polarity. The results obtained in the present study indicated the whole plant V.cinerea as a rich source of phytonutrients, phytochemicals and minerals has medicinal property and it can be used as dietary source.

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