Introduction: Dental caries is the most common childhood illness with nearly half of children aged 2–19 have had dental caries in their teeth. Fluoride varnishes prevents dental caries by increasing remineralization. Aim: To determine substantive fluoride release from 3 different fluoride varnishes over a period of 6 months time. Materials and Methods: Sixty extracted human deciduous anterior teeth were divided into four groups, i.e., ClinproTM XT, Flouritop SR, Flourprotector and Control group. Fluoride varnishes were applied on 3mm x 3mm window on labial surface of the teeth and then the teeth were immersed and stored in artificial saliva. The concentration of fluoride in ppm was measured for 4 hours, 8 hours,1 month, 3 months and 6 months. Fluoride release at each time interval for different groups was statistically analyzed using ANOVA and Tukey’s Kramer test. Results: All the fluoride varnishes released fluoride for period of 6 months, with greatest release observed during 1st 8 hours Fluoritop SR, ClinproTM XT Varnish released consistently and substantially more fluoride than Fluoritop SR and Fluorprotector during 6 months analysis (p<0.05). Conclusion: Over a period of 6 months Clinpro- XT Varnish released consistently and substantially more than other tested products.
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