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A fast scanning stripping square wave voltammetry analysis of erythromycin a in tilapia (oreochromis niloticus) with the dropping mercury electrode

Author: 
Nguyen Phuoc Minh
Subject Area: 
Life Sciences
Abstract: 

A new sensitive analytical approach for determination of erythromycin A in tilapia (Oreochromis niloticus) by a fast scanning stripping square wave voltammetry using the dropping mercury electrode (PSA-F) was developed and validated. Optimum parameters for erythromycin A quantification were: Ammonium acetate buffer 0.1 M, pH 8.0 as supporting electrolyte; acetonitrile as the solvents for disolving erythromycin standard; forward scanning; Vstart: -400 mV; Vstop: -1700 mv; Vstep: 6 mV; Vpulse: 40 mV; Tdop: 5000 ms; Velectrolide: -1100 mV; Telectrolide: 5s. Peak of erythromycin A appeared at E1/2 = -1430 mV, separated and distinguished with peaks of other antibiotics. This assay showed high recovery (> 85.07 %), high sensitivity (detection limit 0.52 µg/kg), high precision (RSD, 0.8  2.1 %), high accuracy (relative error - RE, 85.07 88.56 %) as well as excellent linearity (r2adjusted = 1.0). Simpler, reagent-saving and time-saving were other advantages of this assay method. An equivalence of analyzing results between PSA-F and LC-MS/MS could be obviously seen.

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