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Genome sequence analysis of DNA-A from okra yellow vein mosaic virus isolates

Author: 
Taware, S.D., Thengane, R. J., Harke, S.N and Taware, A S
Subject Area: 
Life Sciences
Abstract: 

Entire DNA-A of four okra yellow vein mosaic virus (OYVMV) Indian biotypes were isolated from virus infected leaf tissue of okra using PCR amplification. Isolated complete DNA-A from experimental biotypes were cloned and sequenced. DNA-A sequences from all these biotypes were compared and further analyzed using the bioinformatics tools. ClustalW analysis of these sequences confirmed variation into the DNA-A amongst OYVMV isolates. It is observed that out of the AV1 gene which encodes coat protein (CP) is the most conserved region, and AC4 protein the most variable. Results also concluded that there is variability in DNA-A of all four experimental biotypes. There are recombinational host spots within DNA-A of these four biotypes. Among all four experimental biotypes of OYVMV, Madurai biotype was found to be more recombinant and formed by major genetic changes into its DNA-A.

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